Pab Rb x human VEGF Receptor 3 antibody 377660 from United States Biological

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Pab Rb x human VEGF Receptor 3 antibody

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with VEGF Receptor 3 at 0.5ug/ml overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VEGF Receptor 3 at approximately 153kD. The expected band size for VEGF Receptor 3 is at 153kD.
Pab Rb x human VEGF Receptor 3 antibody

VEGF Receptor 3 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml VEGF Receptor 3 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human VEGF Receptor 3 antibody

VEGF Receptor 3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml VEGF Receptor 3 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human VEGF Receptor 3 antibody

VEGF Receptor 3 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml VEGF Receptor 3 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human VEGF Receptor 3 antibody

VEGF Receptor 3 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml VEGF Receptor 3 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Pab Rb x human VEGF Receptor 3 antibody

The Pab Rb x human VEGF Receptor 3 antibody from United States Biological is a Rabbit Polyclonal antibody to FLT4, and fms related receptor tyrosine kinase 4. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Pab Rb x human VEGF Receptor 3 antibody has been shown to work in the following applications: Immunohistochemistry, and Western Blot.

Pab Rb x human VEGF Receptor 3 antibody

Pab Rb x human VEGF Receptor 3 antibody

Pab Rb x human VEGF Receptor 3 antibody

Pab Rb x human VEGF Receptor 3 antibody

Pab Rb x human VEGF Receptor 3 antibody

Pab Rb x human VEGF Receptor 3 antibody from United States Biological

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Pab Rb x human VEGF Receptor 3 antibody from United States Biological

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