Western blot using J0400-01B shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains ~ 20ug of lysate. J0400-01B was used at a 1:250 dilution. The membrane was washed and reacted with IgG (HRP) Pab Gt xRb Exposure time was 1 min. Predicted molecular weight is 134kD.