Western Blot of Cos7, HEK293, MEF and Hela cells left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.

Western Blot analysis of APG5L (arrow) 293 cell lysates (2ug/lane) using 032231. Lane 1: nontransfected Lane 2: transiently transfected with the APG5L gene.

Immunohistochemistry analysis of formalin-fixed paraffin-embedded human cancer tissue using 032231 followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of 032231 for immunohistochemistry BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence (IC) analysis of U251 cells using 032231 (1:25). U251 cells were treated with Chloroquine (50uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG (1:400) was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).