Western Blot analysis of E2F2 expression in transfected 293T cell line by E2F2 polyclonal antibody. Lane 1: E2F2 transfected lysate (47.5kD). Lane 2: Non-transfected lysate.

Proximity Ligation Analysis (PLA) of protein-protein interactions between E2F2 and E2F1 HeLa cells were stained with E2F2 rabbit purified polyclonal 1:1200 and E2F1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.