Pab Rb x human MAP1LC3A (Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1 Light Chain 3-Like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha) antibody 397472 from United States Biological

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Pab Rb x human MAP1LC3A (Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1 Light Chain 3-Like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha) antibody

Figure 1. Western blot analysis of MAP1LC3A using MAP1LC3A. Electrophoresis was performed on a 5-20% SDS- PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with MAP1LC3A at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP1LC3A at approximately 18kD. The expected band size for MAP1LC3A is at 14kD.
Pab Rb x human MAP1LC3A (Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1 Light Chain 3-Like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha) antibody

Figure 2. Immunohistochemistry analysis of MAP1LC3A using MAP1LC3A. MAP1LC3A was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit MAP1LC3A Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human MAP1LC3A (Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1 Light Chain 3-Like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha) antibody

Figure 3. Immunohistochemistry analysis of MAP1LC3A using MAP1LC3A. MAP1LC3A was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml MAP1LC3A Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human MAP1LC3A (Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1 Light Chain 3-Like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha) antibody

Figure 4. Immunohistochemistry analysis of MAP1LC3A using anti- MAP1LC3A. MAP1LC3A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml MAP1LC3A Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human MAP1LC3A (Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1 Light Chain 3-Like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha) antibody

Figure 5. Immunohistochemistry analysis of MAP1LC3A using anti- MAP1LC3A. MAP1LC3A was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml MAP1LC3A Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin- Complex (SABC) with DAB as the chromogen.

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Pab Rb x human MAP1LC3A (Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1 Light Chain 3-Like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha) antibody

The Pab Rb x human MAP1LC3A (Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1 Light Chain 3-Like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha) antibody from United States Biological is a Rabbit Polyclonal antibody to MAP1LC3A. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Pab Rb x human MAP1LC3A (Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1 Light Chain 3-Like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha) antibody has been shown to work in the following applications: EIA, Immunoassay, ELISA, Immunohistochemistry, and Western Blot.

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