Western Blot Analysis: Lambda phosphatase untreated and treated rat brain microsomal tissue lysate was probed with N5380-11 (1ug/ml); untreated (lane 1) and treated (lane 2). Proteins were visualized using an IgG (HRP) Donkey Anti-Rabbit and chemiluminescence detection system. Arrow indicates NR1 (~ 120kD).

Paraffin-embedded normal rat hippocampus tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:50 dilution of N5380-11. Reactivity was detected using the IHC-Select® Detection Kit. Staining pattern appears to be restricted to the cytoplasm. Fig. 1 Low mag. and Fig. 2 High mag.

Immunoprecipitation Analysis: 10ug N5380-11 immunoprecipitated NR1 from 500ug of rat brain microsomal RIPA lysate.