Western Blot analysis of MMP13 expression in transfected 293T cell line using 129751. Lane 1: MMP13 transfected lysate (53.8kD). Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged MMP13 is ~1ng/ml as a capture antibody.

Western Blot analysis of MMP13 over-expressed 293 cell line, cotransfected with MMP13 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 129751 GAPDH (36.1kD) used as specificity and loading control.

Immunoprecipitation of MMP13 transfected lysate using 129751 and Protein A Magnetic Bead, and immunoblotted with MMP13 rabbit polyclonal antibody.