Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF4 and TRAF6 HeLa cells were stained with TRAF4 rabbit purified polyclonal 1:1200 and TRAF6 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.