Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human DR4 (hDR4; +) using DR4 Rabbit mAb.

Flow cytometric analysis of U266 cells using DR4 Rabbit mAb (blue) compared to concentration-matched Rabbit mAb IgG Isotype control (red). Anti-rabbit IgG (H+L), F(ab’)2 Fragment was used as a secondary.

Immunoprecipitation of DR4 from PC-3 cell extracts. Lane 1 represents 10% input, lane 2 is Rabbit mAb IgG Isotype Control, and lane 3 is DR4 Rabbit mAb. Western blot was performed using DR4 Rabbit mAb. A conformation specfic secondary antibody was used to avoid IgG cross-reactivity.

Confocal immunofluorescent analysis of HCT 116 (positive, left) and FaDu (negative, right) cells using DR4 Rabbit mAb (green). Blue pseudocolor (fluorescent DNA dye).