Western blot analysis of anti-SPHK1 Antibody (Center) in 293 cell line lysates (35ug/lane). SPHK1 (arrow) was detected using the purified Pab.

Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.