M4696-11 detects a ~250kD band corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24hrs prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100ng/ml) for 20 min prior to harvest. Follow reaction of antibody with IgG (HRP) Gt xRb (1:200) for visualization.
M4696-11 was used (5ug/ml) to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. Tissue was FFPE. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Supplier Page from United States Biological for Rabbit Anti-mTOR, phosphorylated Antibody