Immunoprecipitation of GSK-3β from PC-12 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control (lane 2) or GSK-3β (D5C5Z) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using GSK-3β (D5C5Z) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GSK-3β (D5C5Z) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded MEF cell pellets, wild type (left), GSK-3α (-/-) (middle) and GSK-3β (-/-) (right) using GSK-3β (D5C5Z) XP® Rabbit mAb. (MEF wild type, GSK-3β (-/-), and GSK-3α (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Flow cytometric analysis of wild type mouse embryonic fibroblasts (MEFs) (green) and GSK-3β (-/-) MEFs (blue) using GSK-3β (D5C5Z) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) was used as a secondary antibody. (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).