Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody 397409 from United States Biological

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Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Figure 1. Western blot analysis of FABP5 using FABP5. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with FABP5 at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FABP5 at approximately 15kD. The expected band size for FABP5 is at 15kD.
Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Figure 2. Immunohistochemistry analysis of FABP5 using FABP5. FABP5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit FABP5 Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Figure 3. Immunohistochemistry analysis of FABP5 using FABP5. FABP5 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml FABP5 Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Figure 4. Immunohistochemistry analysis of FABP5 using FABP5. FABP5 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml FABP5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Figure 5. Immunohistochemistry analysis of FABP5 using anti-FABP5. FABP5 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml FABP5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

United States Biological's Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunohistochemistry, and Western Blot. The Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody was generated using FABP5 as the antigen and it reacts with Homo Sapiens, Human, Mouse, and Rat.

Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody

Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody from United States Biological

United States Biological

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Pab Rb x human FABP5 (Fatty Acid-Binding Protein, Epidermal, Epidermal-Type Fatty Acid-Binding Protein, E-FABP, Fatty Acid-Binding Protein 5, Psoriasis-Associated Fatty Acid-Binding Protein Homolog, PA-FABP) antibody from United States Biological

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