Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody 377314 from United States Biological

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Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: PANC-1 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with CPI17 alpha at 0.5ug/ml overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CPI17 alpha at approximately 20kD. The expected band size for CPI17 alpha is at 20kD.
Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

CPI17 alpha was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml CPI17 alpha overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

CPI17 alpha was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml CPI17 alpha overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

CPI17 alpha was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml CPI17 alpha overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)with DAB as the chromogen.
Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

CPI17 alpha was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml CPI17 alpha overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

United States Biological's Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunohistochemistry, and Western Blot. The Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody was generated using PPP1R14A as the antigen and it reacts with Homo Sapiens, Human, Mouse, and Rat.

Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody

Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody from United States Biological

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Pab Rb x human CPI17 alpha (Protein Phosphatase 1 Regulatory Subunit 14A) antibody from United States Biological

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