Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human complexin-1 (hCPLX-1-Myc; +) or complexin-2 (hCPLX- 2-Myc; +), using Complexin-1/2 Rabbit mAb (upper), and Myc-Tag Rabbit mAb (lower).

Confocal immunofluorescent analysis of Neuro-2a (left) and NIH/3T3 (right) cells, using Complexin-1/2 Rabbit mAb (green). Blue pseudocolor = (fluorescent DNA dye).

Immunoprecipitation of complexin-1/2 from Neuro-2A cell extracts. Lane 1 is 10% input, lane 2 is Rabbit mAb IgG Isotype Control and lane 3 is Complexin-1/2 Rabbit mAb. Western blot analysis was performed using Complexin-1/2 Rabbit mAb.