Peptide Inhibition Assay Analysis: Representative lot data. RAT2 cells transfected with Src were probed with phospho-FAK (Tyr397) (1:1,000 dilution). Lane 1: Antibody incubated without peptide Lane 2: Antibody incubated with Non-Phospho peptide Lane 3: Antibody incubated with Phospho peptide Proteins were visualized using a Donkey Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates FAK (~125kD).