Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue using 042602 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates 042602 for immunohistochemistry.

Fluorescent confocal image of Hela cell stained with 042602. Hela cells were fixed with 4% PFA (20min), permeabilized with Triton X-100 (0.1%, 10min), then incubated with 042602 (1:25, 1hr at 37ºC). For secondary antibody, Alexa Fluor®488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50min at 37ºC). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1hr at 37ºC). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10min). TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.