Western Blot analysis in human brain whole cell lysate (20ug/lane) using 036011 (1:1000). Secondary: IgG (HRP) goat anti-rabbit (1:10,000). Predicted band size: 45kD Blocking/Dilution buffer: 5% NFDM/TBST.

Western Blot analysis of MDA-MB435 cell line lysates (20ug/lane) using 036011 (1:100) Secondary: IgG (HRP) goat anti-rabbit (1:10,000). Predicted band size: 45kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis in formalin fixed paraffin embedded human brain tissue using 036011 followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of 036011 for immunohistochemistry.

Flow Cytometric analysis of MDA-MB435 cells using 036011 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.