All lanes: B2185-02B1 (1:2000) Lane 1: 293 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: U-2OS whole cell lysate Lysates/proteins at 20ug/lane. Secondary goat anti-rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 37kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human breast carcinoma reacted with B2185-02B1, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of B2185-02B1 for immunohistochemistry.

Confocal immunofluorescent analysis of MCF-7 cells using B2185-02B1 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Flow Cytometric analysis of MCF-7 cells using B2185-02B1 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.