Immunohistochemistry image of CD34 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.