Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with either a construct expressing DDK-tagged full-length human malic enzyme (cytoplasmic) (hME1-DDK; +) or a construct expressing DDK-tagged full-length human malic enzyme (mitochondrial) (hME2-DDK; +), using Malic Enzyme (Mitochondrial) Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb (middle), or β-Actin (D6A8) Rabbit mAb (lower).

Immunoprecipitation of 137465 from 293 cell extracts, using Normal Rabbit IgG (lane 2) or 137465 (lane 3). Lane 1 is 10% input. Western blot analysis was performed using 137465.