Immunohistochemistry analysis of AMH in paraformaldehyde-fixed, paraffin-embedded human cerebellum tissue sections using 031835 (1:25). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer. pH 6. Samples were incubated with 031835 (1:25) for 1 hours at 37°C. An undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Overlay histogram showing SH-SY5Y cells using 031835 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (031835, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1:200) for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.