Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 polyclonal antibody. Lane 1: HSPB1 transfected lysate (22.8kD). Lane 2: Non-transfected lysate.

Proximity Ligation Analysis (PLA) of protein-protein interactions between HSPB1 and EGFR. HeLa cells were stained with HSPB1 rabbit purified polyclonal 1:1200 and EGFR mouse purified polyclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

Proximity Ligation Analysis (PLA) of protein-protein interactions between HSPB1 and F13A1. Huh7 cells were stained with HSPB1 rabbit purified polyclonal 1:1200 and F13A1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.