Western Blot analysis using 036539 (1:2000) Lane 1: human pancreas lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG (HRP) Goat Anti-Rabbit. Predicted band size: 47kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis in formalin fixed paraffin embedded human colon carcinoma using 036539 followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of 036539 for immunohistochemistry.

Immunohistochemistry (FFPE) analysis of HHLA2 in human pancreas tissue sections using 036539. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with 036539 (1:25) for 1 hour at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.