Lane 1: Detects GST-hSAP30L fusion protein in NIH3T3 cells. Lane 2: Detects wild-type transfected hSAP30L and endogenous hSAP30L in HEK293 cells. Lane 3: Detects endogenous hSAP30L in non-transfected MCF-7 cells. Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein as well as endogenous hSAP30L in human cell lines (e.g., HEK293 and MCF-7 cells). Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein in non-human cell lines (e.g. NIH3T3 cells), but does not detect endogenous non-human SAP30L. The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kD and the endogenous hSAP30L band runs with an apparent molecular wieght of 21-22kD. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be due to cleavage product, degradation and/or product or post-translational modification.