Western blot analysis of SIRT5, pAb: Lane 1: MW marker, Lane 2: SIRT5 (human), (recombinant) (His-tag), Lane 3: SIRT3 (human), (recombinant) (His-tag), Lane 4: SIRT2 (human), (recombinant) (His-tag), Lane 5: SIRT1 (human), (recombinant) (His-tag).

Western blot analysis of SIRT5, pAb: Lane 1: MW marker, Lane 2: SIRT5 (human), (recombinant) (His-tag), Lane 3: SIRT3 (human), (recombinant) (His-tag), Lane 4: SIRT2 (human), (recombinant) (His-tag), Lane 5: SIRT1 (human), (recombinant) (His-tag).

Anti-Pou5f1 Antibody (N-terminal) at 1:2000 dilution + F9 whole cell lysates Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution Predicted band size: 38kD Blocking/Dilution buffer: 5% NFDM/TBST.

Staining (Mouse) Pou5f1 in mouse brain sections by Immunohistochemistry (Immunohistochemistry (paraffin) - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.