The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: RASSF1A Transfected 293T cell lysate

ICC/IF analysis of RASSF1A in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).