Western blot analysis of extracts of various cell lines, using POU5F1B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 90s.
Supplier Page from United States Biological for Pab Rb x human POU5F1B antibody