All lanes : Anti-CDKN1A Antibody (C-terminal) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18kD Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of A549 cells stained with CDKN1A Antibody (C-terminal). CDKN1A was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). Cytoplasm

Western blot analysis of lysates from Hela, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate(from left to right), using CDKN1A Antibody (C-terminal). CDKN1A was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.