Western Blotting Analysis: Representative lot data. Forskolin untreated (Lane 1 and Lane 3) and treated (Lane 2 and Lane 4) PC12 cell lysates were probed with Anti-phospho PKA-RIIalpha (Ser96) (1ug/ml). Phospho specifity was determined by Lambda-phosphatase treatment of lysates in lanes 3 and 4. All proteins were visualized using a Donkey-Anti-Rabbit IgG secondary antibody conjugated to HRPand detected using a chemiluminescence detection system Arrow indicates PKA-RIIalpha (Ser96) (~51kD).