Western Blot showing detection of 0.1ug of recombinant VlsE protein. Lane 1: Molecular weight markers. Lane 2: MBP-VlsE fusion protein (arrow; expected MW: 78.8kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45um nitrocellulose. After blocking with 1% BSA-TTBS diluted to 1X overnight at 4°C, 168587 (1:1000) at RT for 30 min. IgG (HRP goat-rabbit secondary was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system.