Detection of Adiponectin Receptor 1 (human) in HEK 293T transfected cells by A0886-75. 10ug per lane of total cell extracts from mock transfected HEK 293T cells (lanes 1 and 3) or HEK 293T cells transfected with an expression vector encoding the Flag-tagged human AdipoR1 (lanes 2 and 4). Lanes 1 and 2 were probed with an anti-FLAG antibody at a 1:2000 dilution. Lanes 3 and 4 were probed with A0886-75 at a 1:1000 dilution. After 1 hour of incubation at RT, the membrane was extensively washed and then incubated with the appropriate HRP conjugated secondary antibody. Note that the upper bands might represent oligomerized forms of the receptor.