lWestern Blot analysis using 041972 (1:1000) Lane 1: Human lung lysate Lane 2: Human breast lysate Lysates/proteins (20 ug/lane). Secondary IgG (H+L) (HRP) goat anti-rabbit IgG, (H+L), (1:10,000) . Predicted band size: 80kD Blocking/Dilution buffer: 5% NFDM/TBST.

Western Blot analysis in HL-60 cell line lysates (35ug/lane) using 041972. This demonstrates that 041972 detected the SLC6A14 protein (arrow).

Immunohistochemistry analysis in formalin fixed, paraffin embedded human lung tissue using 042972. Tissue was fixed with formaldehyde at RT, antigen retrieval was by heat mediation with a EDTA buffer, pH 9. 0. Samples were incubated with 041972 (1;500) for 1 hour at RT. A undiluted biotinylated CRF anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.