Western Blot analysis using 037746 (1:2000) Lane 1: Hela whole cell lysate Lane 2: Human plasma lysate Lane 3: Mouse lung lysate Lysates/proteins at 20ug/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10.000). Predicted band size: 65kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of paraffin-embedded human prostate tissue using 037746 was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at RT. Heat induced epitope retrieval was performed by EDTA buffer, pH9. 0. Samples were incubated with 037746 (1:100) for 15 minutes at RT..