Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody 397600 from United States Biological

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Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody

Figure 1. Western blot analysis of VCAM1 using VCAM1. Electrophoresis was performed on a 5-20% SDS- PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with VCAM1 at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VCAM1 at approximately 110kD. The expected band size for VCAM1 is at 81kD.
Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody

Figure 2. Immunohistochemistry analysis of VCAM1 using VCAM1. VCAM1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit VCAM1 Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin- Complex (SABC) with DAB as the chromogen.
Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody

Figure 3. Immunohistochemistry analysis of VCAM1 using VCAM1. VCAM1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml VCAM1 Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin- Complex (SABC) with DAB as the chromogen.

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Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody

United States Biological's Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Immunohistochemistry, and Western Blot.

Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody

Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody

Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody

Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody from United States Biological

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Pab Rb x rat Vcam1 (Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, Vcam-1) antibody from United States Biological

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