Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody 397445 from United States Biological

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Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody

Figure 1. Western blot analysis of IGFBP1 using IGFBP1. Electrophoresis was performed on a 5-20% SDS- PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with IGFBP1 at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 30kD. The expected band size for IGFBP1 is at 30kD.
Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody

Figure 2. Immunohistochemistry analysis of IGFBP1 using IGFBP1. IGFBP1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit IGFBP1 Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody

The Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody from United States Biological is a Rabbit Polyclonal antibody to IGFBP1. This antibody recognizes Homo Sapiens, and Human antigen. The Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody has been shown to work in the following applications: Immunohistochemistry, and Western Blot.

Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody

Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody

Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody from United States Biological

United States Biological

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Pab Rb x human IGFBP1 (Insulin-Like Growth Factor-Binding Protein 1, IBP-1, IGF-Binding Protein 1, IGFBP-1, Placental Protein 12, PP12, IBP1) antibody from United States Biological

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