Overlay histogram showing K562 using 036992 (1:25) (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by 036992 (1:25) for 60min at 37ºC. The secondary antibody Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1:200) for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.