Western Blot analysis of BIRC3 expression in transfected 293T cell line by BIRC3 polyclonal antibody. Lane 1: BIRC3 transfected lysate (68.4kD). Lane 2: Non-transfected lysate.

Proximity Ligation Analysis (PLA) of protein-protein interactions between BIRC3 and CASP9 HeLa cells were stained with BIRC3 rabbit purified polyclonal 1:1200 and CASP9 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.