Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody 397502 from United States Biological

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Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Figure 1. Western blot analysis of NSF using NSF. Electrophoresis was performed on a 5-20% SDS- PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human HepG2 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with NSF at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NSF at approximately 82kD. The expected band size for NSF is at 82kD.
Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Figure 2. Immunohistochemistry analysis of NSF using NSF. NSF was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit NSF Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Figure 3. Immunohistochemistry analysis of NSF using NSF. NSF was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml NSF Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Figure 4. Immunohistochemistry analysis of NSF using anti-NSF. NSF was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml NSF Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Figure 5. Immunohistochemistry analysis of NSF using anti-NSF. NSF was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml NSF Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin- Complex (SABC) with DAB as the chromogen.

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Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

United States Biological's Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Immunohistochemistry, and Western Blot. The Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody was generated using Nsf as the antigen and it reacts with Homo Sapiens, Human, Mouse, and Rat.

Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody

Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody from United States Biological

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Pab Rb x human NSF (Vesicle-Fusing ATPase, N-Ethylmaleimide-Sensitive Fusion Protein, NEM-Sensitive Fusion Protein, Vesicular-Fusion Protein NSF) antibody from United States Biological

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