Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded purkinje cells at the junction of the granular and molecular layers of normal rat cerebellum tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:1500 dilution of Anti-InsP3R, Type 1. Reactivity was detected using the IHC-Select® Detection Kit Staining pattern appears as cytoplasmic. Also note the staining of dendritic branches observed in the molecular layer.