Western Blot of 036916 (1:2000) + human plasma lysate Lysates/proteins at 20µg/lane. Secondary IgG (H+L) (HRP)goat anti-rabbit IgG, (H+L), (1;10,000). Predicted band size : 36kD Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HL-60 cells stained with 036916 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by 036916) for 60 min at 37ºC. The secondary antibody used was goat-anti-rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) (1:400) for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.