Western blot analysis of extracts of various cell lines, using TMED2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 3min.
Immunofluorescence analysis of C6 cells using TMED2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using TMED2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Supplier Page from United States Biological for Pab Rb x human TMED2 antibody