Fluorescent confocal image of HeLa cells stained with 037528. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 037528 (1:100, 2h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25μM, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm.

Fluorescent confocal image of SY5Y cells stained with 037528. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 037528 (1:100, 2h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25μM, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei.

Immunofluorescent analysis of U251 cells, using 037528. 037528 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).