Western blot analysis of extracts of various cell lines, using Caspase 8 antibody at 1:1000 dilution. Secondary antibody: HRP Goat AntiRabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry of paraffin embedded human colon carcinoma using Caspase 8 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin embedded mouse kidney using Caspase 8 antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using Caspase 8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Supplier Page from United States Biological for Pab Rb x human Caspase 8 antibody