All lanes: Anti-CLN3 Antibody (Center) at 1:500 dilution Lane 1: Mouse kidney lysate Lane 2: Neuro-2a whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size: 48kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded H. liver section using CLN3 Antibody (Center). CLN3 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Flow cytometric analysis of Hela cells using CLN3 Antibody (Center)(green) compared to an isotype control of rabbit IgG(blue). CLN3 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from A431, KG-1, SH-SY5Y cell line, mouse kidney, mouse liver tissue (from left to right), using CLN3 Antibody (Center). CLN3 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.