Pab Rb x human Integrin alpha 5 antibody 377460 from United States Biological

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Pab Rb x human Integrin alpha 5 antibody

Electrophoresis was performed on a 5-20% SDS- PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human HepG2 cell lysate, Lane 3: rat liver tissue lysate, Lane 4: mouse liver tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with Integrin alpha 5 at 0.5ug/ml overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Integrin alpha 5 at approximately 150kD. The expected band size for Integrin alpha 5 is at 114kD.
Pab Rb x human Integrin alpha 5 antibody

Integrin alpha 5 was detected in paraffin- embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml Integrin alpha 5 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human Integrin alpha 5 antibody

Integrin alpha 5 was detected in paraffin- embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml Integrin alpha 5 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human Integrin alpha 5 antibody

Integrin alpha 5 was detected in paraffin- embedded section of mouse cardiac muscle tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml Integrin alpha 5 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human Integrin alpha 5 antibody

Integrin alpha 5 was detected in paraffin-embedded section of rat cardiac muscle tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml Integrin alpha 5 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Pab Rb x human Integrin alpha 5 antibody

The Pab Rb x human Integrin alpha 5 antibody from United States Biological is a Rabbit Polyclonal antibody to ITGA5. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Pab Rb x human Integrin alpha 5 antibody has been shown to work in the following applications: EIA, Immunoassay, ELISA, Immunohistochemistry, and Western Blot.

Pab Rb x human Integrin alpha 5 antibody

Pab Rb x human Integrin alpha 5 antibody

Pab Rb x human Integrin alpha 5 antibody

Pab Rb x human Integrin alpha 5 antibody

Pab Rb x human Integrin alpha 5 antibody

Pab Rb x human Integrin alpha 5 antibody from United States Biological

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Pab Rb x human Integrin alpha 5 antibody from United States Biological

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