Western Blot Analysis: HeLa lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with A0901-80F(1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein ARF1 (~21kD).

Western Blot Analysis: Representative image from a previous lot. NIH/3T3 lysate (Lane 1), C2C12 lysate (Lane 2), PC12 lysate (Lane 3), A431 lysate (Lane 4), HeLa lysate (Lane 5) and PC3 lysate (Lane 6) were resolved by electrophoresis, transferred to PVDF membrane and probed with A0901-80F(1:500 dilution of a previous lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.Arrow indicates protein ARF1 (~21kD).