Pab Rb x rat MMP13 antibody 377510 from United States Biological

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Pab Rb x rat MMP13 antibody

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel /90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat RH35 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with MMP13 at 0.5ug/ml overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP13 at approximately 54kD. The expected band size for MMP13 is at 54kD.
Pab Rb x rat MMP13 antibody

MMP13 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml MMP13 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)with DAB as the chromogen.
Pab Rb x rat MMP13 antibody

MMP13 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml MMP13 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)with DAB as the chromogen.
Pab Rb x rat MMP13 antibody

MMP13 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml MMP13 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)with DAB as the chromogen.
Pab Rb x rat MMP13 antibody

MMP13 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml MMP13 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Pab Rb x rat MMP13 antibody

The Pab Rb x rat MMP13 antibody from United States Biological is a Rabbit Polyclonal antibody to MMP13. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Pab Rb x rat MMP13 antibody has been shown to work in the following applications: EIA, Immunoassay, ELISA, Immunohistochemistry, and Western Blot.

Pab Rb x rat MMP13 antibody

Pab Rb x rat MMP13 antibody

Pab Rb x rat MMP13 antibody

Pab Rb x rat MMP13 antibody

Pab Rb x rat MMP13 antibody

Pab Rb x rat MMP13 antibody from United States Biological

United States Biological

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Pab Rb x rat MMP13 antibody from United States Biological

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