Western blot analysis of extracts of MEFs from wild-type (-) and OPA knockout (OPA-/-; +) mice using OPA1 Rabbit mAb (upper) or B-Actin Rabbit mAb (lower).

Western blot analysis of extracts from MCF7 and HeLa cells, un- treated (-) or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP; +) using OPA1 Rabbit mAb.

Western blot analysis of extracts from HeLa cells, transfected with 100nM Control siRNA (Unconjugated) (-), OPA1 siRNA I (+), or OPA1 siRNA II using OPA1 Rabbit mAb (upper) or beta-Actin Rabbit mAb (lower). The OPA1 Rabbit mAb confirms silencing of OPA1 expression, while the beta-Actin Rabbit mAb is used as a loading control.

Immunoprecipitation of OPA1 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit mAb IgG Isotype Control, and lane 3 is OPA1 Rabbit mAb. Western blot analysis was performed using OPA1 Rabbit mAb. A conformation specific secondary antibody was used to avoid reactivity with IgG.