Western blot analysis of extracts of U-251MG cells, using OTUD7A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 30s.
Supplier Page from United States Biological for Pab Rb x OTUD7A antibody